ACAT1 Monoclonal antibody

• Home
• Products
• Primary Antibodies
• ACAT1 Monoclonal antibody (60485-4-Ig)

• 

  WB analysis using 60485-4-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60485-4-Ig (ACAT1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60485-4-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60485-4-Ig (ACAT1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

ACAT1 Monoclonal Antibody for WB, ELISA

Cat No. 60485-4-Ig

Clone No.1F3H1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ACAT, Acetoacetyl CoA thiolase, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase, mitochondrial, EC:2.3.1.9

---

Tested Applications

• Positive WB detected in: LNCaP cells, HSC-T6 cells, 4T1 cells, rabbit brain tissue, pig brain tissue, A549 cells, HepG2 cells, MJ cells, Karpas-422 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60485-4-Ig targets ACAT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9219

  Product name: Recombinant human ACAT1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-162 aa of BC010942

  Sequence: MAVLPALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQIKQETGSLAKICCHVRR

• Full Name: acetyl-Coenzyme A acetyltransferase 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC010942
• Gene Symbol: ACAT1
• Gene ID (NCBI): 38
• RRID: AB_3743217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P24752
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.