ACAT2 Monoclonal antibody, PBS Only

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  WB analysis using 68005-1-Ig (same clone as 68005-1-PBS)

  Various cell lysates were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68005-1-Ig (same clone as 68005-1-PBS)

  WB result of ACAT2 antibody (68005-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT2 transfected HepG2 cells. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 68005-1-Ig (same clone as 68005-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68005-1-Ig (same clone as 68005-1-PBS)

  Brain tissues were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68005-1-Ig (same clone as 68005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68005-1-Ig (ACAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68005-1-Ig (same clone as 68005-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAT2 antibody (68005-1-Ig, Clone: 1F5F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68005-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ACAT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68005-1-PBS

Clone No.1F5F11

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ACAT2, ACTL

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Product Information

68005-1-PBS targets ACAT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6596

  Product name: Recombinant human ACAT2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-397 aa of BC000408

  Sequence: MNAGSDPVVIVSAARTIIGSFNGALAAVPVQDLGSTVIKEVLKRATVAPEDVSEVIFGHVLAAGCGQNPVRQASVGAGIPYSVPAWSCQMICGSGLKAVCLAVQSIGIGDSSIVVAGGMENMSKAPHLAYLRTGVKIGEMPLTDSILCDGLTDAFHNCHMGITAENVAKKWQVSREDQDKVAVLSQNRTENAQKAGHFDKEIVPVLVSTRRGLIEVKTDEFPRHGSNIEAMSKLKPYFLTDGTGTVTPANASGINDGAAAVVLMKKSEADKRGLTPLARIVSWSQVGVEPSIMGIGPIPAIKQAVTKAGWSLEDVDIFEINEAFAAVSAAIVKELGLNPEKVNIEGGAIALGHPLGASGCRILVTLLHTLERMGRSRGVAALCIGGGMGIAMCVQRE

• Full Name: acetyl-Coenzyme A acetyltransferase 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC000408
• Gene Symbol: ACAT2
• Gene ID (NCBI): 39
• RRID: AB_2918752
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9BWD1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.