ACBD5 Polyclonal antibody

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  WB analysis using 21080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21080-1-AP (ACBD5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 21080-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21080-1-AP (ACBD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 21080-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 21080-1-AP (ACBD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 21080-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21080-1-AP (ACBD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACBD5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21080-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KIAA1996, Acyl-CoA-binding domain-containing protein 5

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Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue, L02 cells, MCF-7 cells
• Positive IHC detected in: human ovary cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

21080-1-AP targets ACBD5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15287

  Product name: Recombinant human ACBD5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 129-461 aa of BC030555

  Sequence: DLGNVLTSTPNAKTVNGKAESSDSGAESEEEEAQEEVKGAEQSDNDKKMMKKSADHKNLEVIVTNGYDKDGFVQDIQNDIHASSSLNGRSTEEVKPIDENLGQTGKSAVCIHQDINDDHVEDVTGIQHLTSDSDSEVYCDSMEQFGQEESLDSFTSNNGPFQYYLGGHSSQPMENSGFREDIQVPPGNGNIGNMQVVAVEGKGEVKHGGEDGRNNSGAPHREKRGGETDEFSNVRRGRGHRMQHLSEGTKGRQVGSGGDGERWGSDRGSRGSLNEQIALVLMRLQEDMQNVLQRLQKLETLTALQAKSSTSTLQTAPQPTSQRPSWWPFEMSP

• Full Name: acyl-Coenzyme A binding domain containing 5
• Calculated Molecular Weight: 534 aa, 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC030555
• Gene Symbol: ACBD5
• Gene ID (NCBI): 91452
• RRID: AB_2878808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T8D3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACBD5, also known as RDLKD. It is expected to be located in the peroxisome membrane, which is ubiquitinated in brain and kidney tissue. This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. The molecular weight of ACBD5 is 60 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Biol Chem; Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.; Authors - Pamela A Young
mouse	WB	Mol Cell Biochem; Differential distribution of peroxisomal proteins points to specific roles of peroxisomes in the murine retina.; Authors - Yannick Das
human	WB	bioRxiv; The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy; Authors - Iris D Montes