ACBP/DBI Polyclonal antibody

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• ACBP/DBI Polyclonal antibody (32990-1-AP)

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  WB analysis using 32990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32990-1-AP (Acbp/Dbi antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ACBP/DBI Polyclonal Antibody for WB, ELISA

Cat No. 32990-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

ACBP, Acyl-CoA-binding protein, DBI, Diazepam-binding inhibitor, Endozepine

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32990-1-AP targets ACBP/DBI in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg2462

  Product name: Recombinant Mouse ACBP/DBI protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 1-87 aa of NM_007830.4

  Sequence: MSQAEFDKAAEEVKRLKTQPTDEEMLFIYSHFKQATVGDVNTDRPGLLDLKGKAKWDSWNKLKGTSKESAMKTYVEKVDELKKKYGI

• Full Name: diazepam binding inhibitor
• Calculated Molecular Weight: 10KDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: NM_007830.4
• Gene Symbol: Dbi
• Gene ID (NCBI): 13167
• RRID: AB_3742814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P31786
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acbp/Dbi (acyl-CoA binding protein/Diazepam-binding inhibitor) is a widely expressed protein that binds long-chain fatty acyl-CoA esters and plays a role in fatty acyl-CoA transport and pool formation. Acbp/Dbi is critical to cellular proliferation, differentiation, mitochondrial functions, and autophagy. Deletion of Acbp in mouse results in sebocyte hyperplasia and sparse, matted hair with a greasy appearance (PMID: 32632162, 16902415).