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ACC1 Monoclonal antibody
ACC1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Human, Mouse, Rat and More (3)
WB, IHC, IF, ELISA
Cat no : 67373-1-Ig
|Positive WB detected in||HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:10000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
67373-1-Ig targets ACC1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Tested Reactivity||Human, Mouse, Rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||ACC1 fusion protein Ag17503|
|Full Name||acetyl-Coenzyme A carboxylase alpha|
|Calculated molecular weight||2383 aa, 275 kDa|
|Observed molecular weight||250-270 kDa|
|GenBank accession number||BC137287|
|Gene ID (NCBI)||31|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.
|Product Specific Protocols|
|WB protocol for ACC1 antibody 67373-1-Ig||Download protocol|
|IHC protocol for ACC1 antibody 67373-1-Ig||Download protocol|
|IF protocol for ACC1 antibody 67373-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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