ACC1 Monoclonal antibody

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• KD/KO Validated

ACC1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

CloneNo.

1A11G10

Cat no : 67373-1-Ig

Synonyms

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1

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Validation Data Gallery

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  WB analysis using 67373-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67373-1-Ig (ACC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67373-1-Ig

  WB result of ACC1 antibody (67373-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACC1 transfected HeLa cells.

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  WB analysis of NIH/3T3 using 67373-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67373-1-Ig (ACC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67373-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67373-1-Ig (ACC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67373-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67373-1-Ig (ACC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67373-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ACC1 antibody (67373-1-Ig, Clone: 1A11G10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 18 publications below

Product Information

67373-1-Ig targets ACC1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACC1 fusion protein Ag17503
• Full Name: acetyl-Coenzyme A carboxylase alpha
• Calculated molecular weight: 2383 aa, 275 kDa
• Observed molecular weight: 250-270 kDa
• GenBank accession number: BC137287
• Gene symbol: ACACA
• Gene ID (NCBI): 31
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

Publications

Species	Application	Title
mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
human	WB	EMBO J; CARS senses cysteine deprivation to activate AMPK for cell survival.; Authors - Mengqiu Yuan
human	WB	Theranostics; Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription.; Authors - Gengyi Zou
mouse	WB	Phytomedicine; Garlic-derived exosomes regulate PFKFB3 expression to relieve liver dysfunction in high-fat diet-fed mice via macrophage-hepatocyte crosstalk; Authors - Jinfan Liu
human	WB	Front Pharmacol; High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease; Authors - Meina Zou
human	WB	J Agric Food Chem; Capsaicin Attenuates Oleic Acid-Induced Lipid Accumulation via the Regulation of Circadian Clock Genes in HepG2 Cells.; Authors - Run Li