CoraLite®594-conjugated ACC Monoclonal antibody

ACC Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A11G10

Cat no : CL594-67373

Synonyms

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67373-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67373 targets ACC in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ACC fusion protein Ag17503
Full Name acetyl-Coenzyme A carboxylase alpha
Calculated molecular weight 2383 aa, 275 kDa
Observed molecular weight 260-270 kDa
GenBank accession numberBC137287
Gene symbol ACACA
Gene ID (NCBI) 31
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410).

Protocols

Product Specific Protocols
IF protocol for CL594 ACC antibody CL594-67373Download protocol
FC protocol for CL594 ACC antibody CL594-67373Download protocol
Standard Protocols
Click here to view our Standard Protocols