ASIC2 Polyclonal antibody

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  WB analysis of rat brain using 17851-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17851-1-AP (ASIC2 antibody at dilution of 1:300 incubated at 4 degree celsius over night.

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  IP experiment of mouse brain using 17851-1-AP

  IP result of anti-ASIC2 (IP:17851-1-AP, 4ug; Detection:17851-1-AP 1:500) with mouse brain tissue lysate 4000ug.

ASIC2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 17851-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

ACCN, ACCN1, Acid sensing ion channel 2, ASIC2, ASIC2a, BNaC1, BNC1, Brain sodium channel 1, hBNaC1, Mammalian degenerin homolog, MDEG

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Tested Applications

• Positive WB detected in: rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

17851-1-AP targets ASIC2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12301

  Product name: Recombinant human ACCN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 60-426 aa of BC075043

  Sequence: SSERVSYYFSYQHVTKVDEVVAQSLVFPAVTLCNLNGFRFSRLTTNDLYHAGELLALLDVNLQIPDPHLADPSVLEALRQKANFKHYKPKQFSMLEFLHRVGHDLKDMMLYCKFKGQECGHQDFTTVFTKYGKCYMFNSGEDGKPLLTTVKGGTGNGLEIMLDIQQDEYLPIWGETEETTFEAGVKVQIHSQSEPPFIQELGFGVAPGFQTFVATQEQRLTYLPPPWGECRSSEMGLDFFPVYSITACRIDCETRYIVENCNCRMVHMPGDAPFCTPEQHKECAEPALGLLAEKDSNYCLCRTPCNLTRYNKELSMVKIPSKTSAKYLEKKFNKSEKYISENILVLDIFFEALNYETIEQKKAYEVA

• Full Name: amiloride-sensitive cation channel 1, neuronal
• Calculated Molecular Weight: 512 aa, 58 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC075043
• Gene Symbol: ASIC2
• Gene ID (NCBI): 40
• RRID: AB_2878451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16515
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASIC2 (acid-sensing ion channel 2), also known as ACCN1 (amiloride-sensitive cation channel 1, neuronal), is a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. ASIC2 is widely expressed in the central nervous system (CNS), may playing a role in neurotransmission. It has two alternatively spliced variants, ASIC2a and ASIC2b.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Aging (Albany NY); ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration.; Authors - Jingyu Ding
human	WB	Sci Rep; Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons.; Authors - Junyu Xu