ASIC4 Polyclonal antibody

ASIC4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12003-1-AP

Synonyms

ACCN4, Acid sensing ion channel 4, ASIC4, BNAC4

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Validation Data Gallery

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  WB analysis of A375 using 12003-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12003-1-AP (ASIC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12003-1-AP targets ASIC4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASIC4 fusion protein Ag2664
• Full Name: amiloride-sensitive cation channel 4, pituitary
• Calculated Molecular Weight: 647 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC010439
• Gene Symbol: ASIC4
• Gene ID (NCBI): 55515
• RRID: AB_2242283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration.; Authors - Jingyu Ding