ACE2 Polyclonal antibody

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  WB analysis using 31518-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31518-1-AP (ACE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 31518-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31518-1-AP (ACE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 31518-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31518-1-AP (ACE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACE2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31518-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ACE related carboxypeptidase, ACEH, ACE-related carboxypeptidase, Angiotensin converting enzyme 2, Angiotensin-converting enzyme 2

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse small intestine tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31518-1-AP targets ACE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg0074

  Product name: Recombinant Human ACE2 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: N-6*His

  Domain: 18-740 aa of BC048094

  Sequence: QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS

• Full Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Calculated Molecular Weight: 805 aa, 92 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC048094
• Gene Symbol: ACE2
• Gene ID (NCBI): 59272
• RRID: AB_3670016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9BYF1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiotensin-converting enzyme 2 (ACE2), a homolog of angiotensin-converting enzyme 1 (ACE1), was identified in 2000. ACE2 provides protection against several chronic diseases, including cardiovascular diseases, lung injury, and diabetes. Besides chronic diseases, ACE2 plays a crucial role in severe acute respiratory syndrome coronavirus (SARS-CoV) infection and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection (hereafter named COVID-19). ACE2 is the host receptor for SARS-CoV and SARS-CoV-2. (PMID: 37608279)