ACE2 Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP02357-1

  Sandwich ELISA standard curve of MP02357-1, Human ACE2 Recombinant Matched Antibody Pair - PBS only. 80062-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0074. 80062-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

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  Sample test of MP02357-1

  The mean ACE2 concentration was determined to be 24.0 ng/mL in human urine .

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  Sample test of MP02357-1

  Plasma of six individual healthy human donors was measured. The human ACE2 concentration of detected samples was determined to be 0.55 ng/mL with a range of 0.21 - 1.03 ng/mL.

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  Sample test of MP02357-1

  The mean ACE2 concentration was determined to be 1.83 ng/mL in HT-29 cell extract based on a 1.1 mg/mL extract load.

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  Cytometric bead array standard curve of MP02357-1

  Cytometric bead array standard curve of MP02357-1, ACE2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80062-3-PBS. Detection antibody: 80062-2-PBS. Standard: Eg0074. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 80062-3-PBS

  Biolayer interferometry (BLl) kinetic assays of 80062-3-PBS against Human ACE2 were performed. The affinity constant is below 1 pM.

ACE2 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 80062-3-PBS

Clone No.250363D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

ACE related carboxypeptidase, ACEH, ACE-related carboxypeptidase, Angiotensin converting enzyme 2, Angiotensin-converting enzyme 2

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Product Information

80062-3-PBS targets ACE2 as part of a matched antibody pair:

MP02357-1: 80062-3-PBS capture and 80062-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0074

  Product name: Recombinant Human ACE2 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: N-6*His

  Domain: 18-740 aa of BC048094

  Sequence: QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS

• Full Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Calculated Molecular Weight: 805 aa, 92 kDa
• GenBank Accession Number: BC048094
• Gene Symbol: ACE2
• Gene ID (NCBI): 59272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BYF1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.