Product Information
30522-1-PBS targets ACIN1-L specific in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30506 Product name: Recombinant human ACIN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-270 aa of BC140805 Sequence: MWRRKHPRTSGGTRGVLSGNRGVEYGSGRGHLGTFEGRWRKLPKMPEAVGTDPSTSRKMAELEEVTLDGKPLQALRVTDLKAALEQRGLAKSGQKSALVKRLKGALMLENLQKHSTPHAAFQPNSQIGEEMSQNSFIKQYLEKQQELLRQRLEREAREAAELEEASAESEDEMIHPEGVASLLPPDFQSSLERPELELSRHSPRKSSSISEEKGDSDDEKPRKGERRSSRVRQARAAKLSEGSQPAEEEEDQETPSRNLRVRADRNLKTE Predict reactive species |
| Full Name | apoptotic chromatin condensation inducer 1 |
| Calculated Molecular Weight | 1341 aa, 152 kDa |
| Observed Molecular Weight | 240 kDa |
| GenBank Accession Number | BC140805 |
| Gene Symbol | ACIN1 |
| Gene ID (NCBI) | 22985 |
| RRID | AB_3669730 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKV3 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. ACIN1 is a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. There are three isoforms of human acinus (the L, S and S' forms) shows at about 80-85 kDa and 220-250 kDa by Western Blot, which were probably generated by alternative splicing (PMID: 10490026). 30522-1-AP specificly targets at the ACIN1-L, while another antibody 23937-1-AP can detect all three isoforms of ACIN1.
