ACLY Recombinant monoclonal antibody, PBS Only

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• ACLY Recombinant monoclonal antibody, PBS Only (87231-1-PBS)

ACLY Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87231-1-PBS

Clone No.252489F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

ATP citrate lyase, ACL, ATP-citrate, ATP-citrate synthase, EC:2.3.3.8

• 

  WB analysis using 87231-1-RR (same clone as 87231-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87231-1-RR (ACLY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87231-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87231-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87231-1-RR against Human ACLY were performed. The affinity constant is 2.49 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87231-1-PBS targets ACLY in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7709

  Product name: Recombinant human ACLY protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 751-1101 aa of BC006195

  Sequence: MFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

• Full Name: ATP citrate lyase
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC006195
• Gene Symbol: ACLY
• Gene ID (NCBI): 47
• RRID: AB_3745418
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P53396
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461).