ACOT11 Polyclonal antibody

ACOT11 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10776-1-AP

Synonyms

ACOT11, acyl CoA thioesterase 11, BFIT, BFIT1, BFIT2, KIAA0707, STARD14, THEA, THEM1

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Validation Data Gallery

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  WB analysis using 10776-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10776-1-AP (ACOT11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10776-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10776-1-AP (ACOT11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 10776-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10776-1-AP (ACOT11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 10776-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10776-1-AP (ACOT11 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 10776-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ACOT11 antibody (10776-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: DU 145 cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10776-1-AP targets ACOT11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACOT11 fusion protein Ag1176
• Full Name: acyl-CoA thioesterase 11
• Calculated Molecular Weight: 68.4 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC001517
• Gene Symbol: ACOT11
• Gene ID (NCBI): 26027
• RRID: AB_2257798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACOT11(Acyl-coenzyme A thioesterase 11) is also named as BFIT, KIAA0707, THEA.BFIT is mapped to syntenic regions of chromosomes 1 (human) and 4 (mouse) associated with body fatness and diet-induced obesity, potentially linking a deficit of BFIT activity with exacerbation of these traits and supports the transition of this tissue towards increased metabolic activity, probably through alteration of intracellular fatty acyl-CoA concentration(PMID:11696000).