ACOT2 Polyclonal antibody

ACOT2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15633-1-AP

Synonyms

ACOT2, acyl CoA thioesterase 2, CTE Ia, Mte1, PTE2, PTE2A, ZAP128



Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, HepG2 cells, human testis tissue, human brain tissue, human kidney tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue, human skeletal muscle tissue, human heart tissue, human testis tissue, human liver tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15633-1-AP targets ACOT2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOT2 fusion protein Ag8093
Full Name acyl-CoA thioesterase 2
Calculated Molecular Weight 483 aa, 53 kDa
Observed Molecular Weight 46-53 kDa
GenBank Accession NumberBC006335
Gene Symbol ACOT2
Gene ID (NCBI) 10965
RRIDAB_2221535
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxi­dative tissues and is poised to modulate mitochondrial FA oxidation (FAO) (PMID: 25114170). The structure of ACOT2 consists of two domains, N and C domains, and the active site of ACOT2 is located at the interface between the N and C domains (PMID: 19497300).

Protocols

Product Specific Protocols
WB protocol for ACOT2 antibody 15633-1-APDownload protocol
IHC protocol for ACOT2 antibody 15633-1-APDownload protocol
IF protocol for ACOT2 antibody 15633-1-APDownload protocol
IP protocol for ACOT2 antibody 15633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Eur J Immunol

YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.

Authors - Satabdi Nandi
mouseWB

Life Sci Alliance

High levels of TFAM repress mammalian mitochondrial DNA transcription in vivo

Authors - Nina A Bonekamp
mouseWB

Biomed Res Int

Up-Regulated MicroRNA-27b Promotes Adipocyte Differentiation via Induction of Acyl-CoA Thioesterase 2 Expression.

Authors - Yuka Murata
mouseWB

J Biol Chem

Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet

Authors - Ebru S. Selen
mouseWB

EMBO Mol Med

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy

Authors - Nneka Southwell
humanIF

Hepatol Commun

Dysregulation of lipid metabolism in the pseudolobule promotes region-specific autophagy in hepatitis B liver cirrhosis

Authors - Wenjun Pu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lana (Verified Customer) (06-21-2019)

Lines on WB:1. BioRad Precision Plus Protein standards2. Homogenate of human cortex3. Non-synaptosomal mitochondria of human cortex4. Homogenate of mouse brains5. Non-synaptosomal mitochondria of mouse brains6. Whole cell lysate of rat PC12 cells7. Mitochondria of rat C12 cellsNotes: Specific band 48 kDa in human samples only. Strong non-specific band 40 kDa in all samples types.WB protocol:SDS-PAGE: 15 ug/ul of RIPA lysates, 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1 h incubation at room temperature.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissues, human and mouse, rat cell line.
ACOT2 Antibody Western Blot, validation (1:1000 dilution) in Brain tissues, human and mouse, rat cell line. (Cat no:15633-1-AP)