|Positive WB detected in||HEK-293 cells, HepG2 cells, human kidney tissue, human brain tissue, human testis tissue, mouse kidney tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human heart tissue, human kidney tissue, human liver tissue, human ovary tissue, human skeletal muscle tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15633-1-AP targets ACOT2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACOT2 fusion protein Ag8093|
|Full Name||acyl-CoA thioesterase 2|
|Calculated molecular weight||483 aa, 53 kDa|
|Observed molecular weight||46-53 kDa|
|GenBank accession number||BC006335|
|Gene ID (NCBI)||10965|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
|Product Specific Protocols|
|WB protocol for ACOT2 antibody 15633-1-AP||Download protocol|
|IHC protocol for ACOT2 antibody 15633-1-AP||Download protocol|
|IF protocol for ACOT2 antibody 15633-1-AP||Download protocol|
|IP protocol for ACOT2 antibody 15633-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biomed Res Int
Up-Regulated MicroRNA-27b Promotes Adipocyte Differentiation via Induction of Acyl-CoA Thioesterase 2 Expression.
Eur J Immunol
YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lana (Verified Customer) (06-21-2019)
Lines on WB:1. BioRad Precision Plus Protein standards2. Homogenate of human cortex3. Non-synaptosomal mitochondria of human cortex4. Homogenate of mouse brains5. Non-synaptosomal mitochondria of mouse brains6. Whole cell lysate of rat PC12 cells7. Mitochondria of rat C12 cellsNotes: Specific band 48 kDa in human samples only. Strong non-specific band 40 kDa in all samples types.WB protocol:SDS-PAGE: 15 ug/ul of RIPA lysates, 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1 h incubation at room temperature.