ACOT2 Polyclonal antibody

ACOT2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15633-1-AP

Synonyms

ACOT2, acyl CoA thioesterase 2, CTE Ia, Mte1, PTE2, PTE2A, ZAP128



Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, HepG2 cells, human kidney tissue, human testis tissue, mouse kidney tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman heart tissue, human kidney tissue, human liver tissue, human ovary tissue, human skeletal muscle tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

15633-1-AP targets ACOT2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOT2 fusion protein Ag8093
Full Name acyl-CoA thioesterase 2
Calculated molecular weight 483 aa, 53 kDa
Observed molecular weight 46-53 kDa
GenBank accession numberBC006335
Gene symbol ACOT2
Gene ID (NCBI) 10965
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACOT2 antibody 15633-1-APDownload protocol
IHC protocol for ACOT2 antibody 15633-1-APDownload protocol
IF protocol for ACOT2 antibody 15633-1-APDownload protocol
IP protocol for ACOT2 antibody 15633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biomed Res Int

Up-Regulated MicroRNA-27b Promotes Adipocyte Differentiation via Induction of Acyl-CoA Thioesterase 2 Expression.

Authors - Yuka Murata
mouseWB

Eur J Immunol

YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.

Authors - Satabdi Nandi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lana (Verified Customer) (06-21-2019)

Lines on WB: 1. BioRad Precision Plus Protein standards 2. Homogenate of human cortex 3. Non-synaptosomal mitochondria of human cortex 4. Homogenate of mouse brains 5. Non-synaptosomal mitochondria of mouse brains 6. Whole cell lysate of rat PC12 cells 7. Mitochondria of rat C12 cells Notes: Specific band 48 kDa in human samples only. Strong non-specific band 40 kDa in all samples types. WB protocol: SDS-PAGE: 15 ug/ul of RIPA lysates, 4-12% Bis-tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h Primary Ab: O/N incubation at 4C. Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1 h incubation at room temperature.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissues, human and mouse, rat cell line.
ACOT2 Antibody Western Blot, validation (1:1000 dilution) in Brain tissues, human and mouse, rat cell line. (Cat no:15633-1-AP)