ACOT1/2 Recombinant monoclonal antibody

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  WB analysis using 82122-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82122-5-RR (ACOT1/2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 82122-5-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82122-5-RR (ACOT2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 82122-5-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82122-5-RR (ACOT1/2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 82122-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACOT1/2 antibody (82122-5-RR, Clone: 251453E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ACOT1/2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 82122-5-RR

Clone No.251453E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ACOT2, ACOT1, acyl CoA thioesterase 2, Acyl-CoA thioesterase 2, Acyl-coenzyme A thioester hydrolase 2a

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Tested Applications

• Positive WB detected in: L02 cells, U-87 MG cells, HEK-293 cells, HepG2 cells, RT-4 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82122-5-RR targets ACOT1/2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8093

  Product name: Recombinant human ACOT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 222-483 aa of BC006335

  Sequence: IVDMFGTGGGLLEYRASLLAGKGFAVMALAYYNYEDLPKTMETLHLEYFEEAMNYLLSHPEVKGPGVGLLGISKGGELCLSMASFLKGITAAVVINGSVANVGGTLRYKGETLPPVGVNRNRIKVTKDGYADIVDVLNSPLEGPDQKSFIPVERAESTFLFLVGQDDHNWKSEFYANEACKRLQAHGRRKPQIICYPETGHYIEPPYFPLCRASLHALVGSPIIWGGEPRAHVMAQVDAWKQLQTFFHKHLGGHEGTIPSKV

• Full Name: acyl-CoA thioesterase 2
• Calculated Molecular Weight: 483 aa, 53 kDa
• Observed Molecular Weight: 46-53 kDa
• GenBank Accession Number: BC006335
• Gene Symbol: ACOT2
• Gene ID (NCBI): 10965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49753
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxi­dative tissues and is poised to modulate mitochondrial FA oxidation (FAO) (PMID: 25114170). The structure of ACOT2 consists of two domains, N and C domains, and the active site of ACOT2 is located at the interface between the N and C domains (PMID: 19497300). ACOT2 shares 98% homology with ACOT1, and this antibody can detect both ACOT1 and ACOT2.