ACOT9 Polyclonal antibody

ACOT9 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15901-1-AP

Synonyms

ACATE2, ACOT9, Acyl CoA thioester hydrolase 9, acyl CoA thioesterase 9, CGI 16, MT ACT48

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Validation Data Gallery

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  WB analysis using 15901-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACOT9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15901-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15901-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15901-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15901-1-AP (ACOT9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15901-1-AP (ACOT9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, mouse heart tissue, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: mouse kidney tissue, human kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

15901-1-AP targets ACOT9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACOT9 fusion protein Ag8730
• Full Name: acyl-CoA thioesterase 9
• Calculated Molecular Weight: 24 kDa, 49 kDa
• Observed Molecular Weight: 43-50 kDa
• GenBank Accession Number: BC012573
• Gene Symbol: ACOT9
• Gene ID (NCBI): 23597
• RRID: AB_2221519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa.

Publications

Species	Application	Title
human	IHC	Liver Int; Acyl-CoA thioesterase 9 promotes tumor growth and metastasis through reprogramming of fatty acid metabolism in hepatocellular carcinoma; Authors - Bao Wang ; KD Validated
mouse	WB	EMBO Mol Med; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy; Authors - Nneka Southwell