Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tisue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17571-1-AP targets ACOX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11778 Product name: Recombinant human ACOX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-681 aa of BC047700 Sequence: KVLDYQTQQQKLFPQLAISYAFHFLAVSLLEFFQHSYTAILNQDFSFLPELHALSTGMKAMMSEFCTQGAEMCRRACGGHGYSKLSGLPSLVTKLSASCTYEGENTVLYLQVARFLVKSYLQTQMSPGSTPQRSLSPSVAYLTAPDLARCPAQRAADFLCPELYTTAWAHVAVRLIKDSVQHLQTLTQSGADQHEAWNQTTVIHLQAAKVHCYYVTVKGFTEALEKLENEPAIQQVLKRLCDLHAIHGILTNSGDFLHDAFLSGAQVDMARTAYLDLLRLIRKDAILLTDAFDFTDQCLNSALGCYDGNVYERLFQWAQKSPTNTQENPAYEEYIRPLLQSWRSKL Predict reactive species |
| Full Name | acyl-Coenzyme A oxidase 2, branched chain |
| Calculated Molecular Weight | 681 aa, 77 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC047700 |
| Gene Symbol | ACOX2 |
| Gene ID (NCBI) | 8309 |
| RRID | AB_2221803 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99424 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACOX2 (Acyl-CoA Oxidase 2) is a peroxisomal enzyme that catalyzes the first and rate-limiting step of branched-chain fatty acid β-oxidation, specifically targeting very long-chain fatty acids (VLCFAs) and bile acid intermediates. It plays a critical role in lipid metabolism by generating H2O2 and acetyl-CoA, thereby linking peroxisomal fatty acid oxidation to cellular energy homeostasis and redox signaling. Dysregulation of ACOX2 has been implicated in metabolic disorders, including non-alcoholic fatty liver disease (NAFLD) and certain peroxisomal biogenesis disorders, highlighting its potential as a therapeutic target for metabolic and liver-related diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ACOX2 antibody 17571-1-AP | Download protocol |
| IHC protocol for ACOX2 antibody 17571-1-AP | Download protocol |
| IP protocol for ACOX2 antibody 17571-1-AP | Download protocol |
| WB protocol for ACOX2 antibody 17571-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ACOX2 antibody (Cat# 17571-1-AP) has 9 citations published in journals including Molecular Cancer, Cell Death & Disease, Phytomedicine, Biochemical Pharmacology, European Journal of Pharmacology, Hypertension Research, Histopathology, Journal of Cancer, and Food and Chemical Toxicology. Research fields span cancer biology (renal cell, bladder, lung, breast, and prostate cancers), lipid metabolism, fatty acid β-oxidation, mitochondrial homeostasis, oxidative stress, and renal/liver disease.
| Species | Application | Title |
|---|---|---|
Mol Cancer ACOX2 destabilizes the MRE11-RAD50-NBS1 complex and boosts anticancer immunity via the cGAS-STING pathway in clear cell renal cell carcinoma. | ||
Cell Death Dis Cytoskeletal protein KRT14 governs cisplatin resistance by modulating eIF4H-dependent ACOX2 translation and lipid metabolism in bladder cancer. | ||
Phytomedicine Ramulus Mori (Sangzhi) Alkaloids (SZ-A) exert a renoprotective effect by activating PGC-1α to mediate fatty acid oxidation and mitochondrial homeostasis. | ||
Biochem Pharmacol Ubiquitin ligase TRIM63 regulates HOXA10 expression to mediate fatty acid β-oxidation and suppress lung adenocarcinoma metastasis | ||
Eur J Pharmacol Isoastragaloside II modulates PPAR-α/FXR signaling and bile acid metabolism to ameliorate cholestatic liver diseases (CLD). | ||
Hypertens Res Increased expression of acyl-CoA oxidase 2 in the kidney with plasma phytanic acid and altered gut microbiota in spontaneously hypertensive rats. |
