ACOXL Polyclonal antibody

ACOXL Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23366-1-AP

Synonyms

ACOXL, Acyl CoA oxidase like protein, acyl Coenzyme A oxidase like

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Validation Data Gallery

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  IP experiment of K-562 using 23366-1-AP

  IP result of anti-ACOXL (IP:23366-1-AP, 3ug; Detection:23366-1-AP 1:300) with K-562 cells lysate 2000ug.

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  IHC staining of human spleen using 23366-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 23366-1-AP (ACOXL Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 23366-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 23366-1-AP (ACOXL Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IP detected in: K-562 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 23366-1-AP is ACOXL Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACOXL fusion protein Ag18625
• Full Name: acyl-Coenzyme A oxidase-like
• Calculated Molecular Weight: 547 aa, 61 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC131514
• Gene Symbol: ACOXL
• Gene ID (NCBI): 55289
• RRID: AB_2879266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	BMC Complement Med Ther; Salvia-Nelumbinis naturalis improves lipid metabolism of NAFLD by regulating the SIRT1/AMPK signaling pathway; Authors - Yang Liu
human	IHC	Front Genet; Transcriptome profiles of fatty acid metabolism-related genes and immune infiltrates identify hot tumors for immunotherapy in cutaneous melanoma; Authors - Yunxian Dong