ACP5 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• ACP5 Polyclonal antibody (27898-1-AP)

• 

  WB analysis using 27898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27898-1-AP (ACP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ACP5 Polyclonal Antibody for WB, ELISA

Cat No. 27898-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ACP 5, EC:3.1.3.2, Tartrate-resistant acid ATPase, Tartrate-resistant acid phosphatase type 5, TR-AP

---

Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, SW480 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27898-1-AP targets ACP5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27383

  Product name: Recombinant human ACP5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 43-199 aa of BC025414

  Sequence: HTAREMANAKEIARTVQILGADFILSLGDNFYFTGVQDINDKRFQETFEDVFSDRSLRKVPWYVLAGNHDHLGNVSAQIAYSKISKRWNFPSPFYRLHFKIPQTNVSVAIFMLDTVTLCGNSDDFLSQQPERPRDVKLARTQLSWLKKQLAAAREDY

• Full Name: acid phosphatase 5, tartrate resistant
• Calculated Molecular Weight: 325 aa, 37 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC025414
• Gene Symbol: ACP5
• Gene ID (NCBI): 54
• RRID: AB_3742265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13686
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tartrate-resistant acid phosphatase (TRAP/ACP5) is a metalloproteinase enzyme that maps to chromosome 19p13.2 and spans approximately 3 kb, belonging to the acid phosphatase family that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. Accumulating studies have demonstrated that ACP5 broadly participates in various malignant processes including uncontrolled proliferation, chemotherapy resistance, cellular invasion and metastasis.