ACPP Monoclonal antibody, PBS Only

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  WB analysis of K-562 using 60176-1-Ig (same clone as 60176-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 60176-1-Ig (same clone as 60176-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 60176-1-Ig(ACPP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 60176-1-Ig(ACPP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

ACPP Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 60176-1-PBS

Clone No.1G2C9

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

PAP, 1G2C9, 5'-NT, 5'-nucleotidase, Acid phosphatase 3

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Product Information

60176-1-PBS targets ACPP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8578

  Product name: Recombinant human ACPP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 27-373 aa of BC007460

  Sequence: DRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELAGPVIPQDWSTECM

• Full Name: acid phosphatase, prostate
• Calculated Molecular Weight: 418 aa, 48 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC007460
• Gene Symbol: ACPP
• Gene ID (NCBI): 55
• RRID: AB_10733336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15309
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).