ACPP Recombinant monoclonal antibody, PBS Only

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• ACPP Recombinant monoclonal antibody, PBS Only (87842-1-PBS)

ACPP Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 87842-1-PBS

Clone No.253501A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

PAP, 5'-NT, 5'-nucleotidase, Acid phosphatase 3, acid phosphatase, prostate

• 

  WB analysis using 87842-1-RR (same clone as 87842-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87842-1-RR (ACPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87842-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate using 87842-1-RR (same clone as 87842-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate tissue slide using 87842-1-RR (PPAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87842-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate using 87842-1-RR (same clone as 87842-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate tissue slide using 87842-1-RR (PPAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87842-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87842-1-PBS targets ACPP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6542

  Product name: Recombinant human PPAP protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 33-386 aa of NM_001099.5

  Sequence: KELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQGTEDSTD

• Full Name: acid phosphatase, prostate
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: NM_001099.5
• Gene Symbol: ACPP
• Gene ID (NCBI): 55
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15309
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042).