ACRV1 Polyclonal antibody

ACRV1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 14040-1-AP

Synonyms

acrosomal vesicle protein 1, Acrosomal protein SP-10, Acrosomal protein SP 10

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Validation Data Gallery

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  WB analysis of DU 145 using 14040-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 14040-1-AP (ACRV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 14040-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 14040-1-AP (ACRV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14040-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14040-1-AP (ACRV1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of DU 145 using 14040-1-AP

  IP result of anti-ACRV1 (IP:14040-1-AP, 3ug; Detection:14040-1-AP 1:2000) with DU 145 cells lysate 560ug.

• 

  IHC staining of human testis using 14040-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14040-1-AP (ACRV1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 14040-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14040-1-AP (ACRV1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of PC-3 using 14040-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using ACRV1 antibody (14040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: DU 145 cells, LNCaP cells, PC-3 cells
• Positive IP detected in: DU 145 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 12 publications below

Product Information

14040-1-AP targets ACRV1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACRV1 fusion protein Ag5109
• Full Name: acrosomal vesicle protein 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 13-36 kDa
• GenBank Accession Number: BC014588
• Gene Symbol: ACRV1
• Gene ID (NCBI): 56
• RRID: AB_10640426
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein ACRV1, also known as SP-10, is an acrosomal matrix protein, which is evolutionarily conserved among mammals. It is associated with the matrix of the acrosomal vesicle in developing spermatids and the acrosomal matrix and membranes of ejaculated sperm (PMID: 8288254). Human ACRV1 protein has 11 isoforms, and molecular weight ranges from 9 to 28 kDa. ACRV1 was predominantly located in round and elongated spermatids in the testis, indicating that ACRV1 may play an important role in mammalian spermatogenesis and may be a target of a contraceptive vaccine (PMID: 21488928; 8874713).

Publications

Species	Application	Title
mouse	IF	Nat Commun; TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice.; Authors - Deqiang Ding
mouse	IHC	Int J Mol Sci; BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis.; Authors - Chao Huang
rat	IF	Syst Biol Reprod Med; Recellularized rat testis scaffolds with embryoid bodies cells: a promising approach for tissue engineering; Authors - Vitória Frias Batista
mouse	IF	Bioinformatics; SATINN: an automated neural network-based classification of testicular sections allows for high-throughput histopathology of mouse mutants; Authors - Ran Yang
mouse	IF	Nat Commun; SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility; Authors - Jiali Liu
mouse	IF	Arch Toxicol; Extended exposure to tetrabromobisphenol A-bis(2,3-dibromopropyl ether) leads to subfertility in male mice at the late reproductive age; Authors - Yuan-Yuan Li