ACRV1 Antibody

ACRV1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14040-1-AP

Synonyms

Acrosomal protein SP 10, acrosomal vesicle protein 1, ACRV1, D11S4365, SP 10, SPACA2



Tested Applications

Positive WB detected inDU 145 cells, LNCaP cells, PC-3 cells
Positive IP detected inDU 145 cells
Positive IHC detected inhuman testis tissue,
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 2 publications below

Product Information

14040-1-AP targets ACRV1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACRV1 fusion protein Ag5109
Full Name acrosomal vesicle protein 1
Calculated molecular weight 28 kDa
Observed molecular weight 13-36 kDa
GenBank accession number BC014588
Gene symbol ACRV1
Gene ID (NCBI) 56
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACRV1 antibody 14040-1-APDownload protocol
IHC protocol for ACRV1 antibody 14040-1-APDownload protocol
IF protocol for ACRV1 antibody 14040-1-APDownload protocol
IP protocol for ACRV1 antibody 14040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nucleic Acids Res

Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis.

Authors - Deqiang Ding
mouseIF

Nat Commun

TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice.

Authors - Deqiang Ding