ACSF2 Polyclonal antibody

ACSF2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16140-1-AP

Synonyms

ACSF2, ACSMW, AVYV493

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Validation Data Gallery

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  WB analysis of mouse lung using 16140-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16140-1-AP (ACSF2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human colon cancer using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16140-1-AP (ACSF2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16140-1-AP (ACSF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 16140-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 16140-1-AP (ACSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human colon cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

16140-1-AP targets ACSF2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSF2 fusion protein Ag9199
• Full Name: acyl-CoA synthetase family member 2
• Calculated Molecular Weight: 615 aa, 68 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC012053
• Gene Symbol: ACSF2
• Gene ID (NCBI): 80221
• RRID: AB_2878222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.

Publications

Species	Application	Title
rat	WB,IP	Prostaglandins Other Lipid Mediat; Targeted Lipidomics Analysis of Lysine 179 Acetylation of ACSF2 in Rat Hepatic Stellate Cells; Authors - Meili Wang
mouse,rat	WB,IHC	Life Sci; ACSF2-mediated ferroptosis is involved in ulcerative colitis; Authors - Lianxiang Luo
human	WB,IF	Free Radic Biol Med; Inhibition of ACSF2 protects against renal ischemia/reperfusion injury via mediating mitophagy in proximal tubular cells; Authors - Haoyu Shi ; KD Validated