ACSF2 Polyclonal antibody

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  WB analysis of mouse lung using 16140-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16140-1-AP (ACSF2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human colon cancer using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16140-1-AP (ACSF2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16140-1-AP (ACSF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 16140-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 16140-1-AP (ACSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ACSF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16140-1-AP

Publications(10)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human colon cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16140-1-AP targets ACSF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9199

  Product name: Recombinant human ACSF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 336-615 aa of BC012053

  Sequence: FNGKKALEAISRERGTFLYGTPTMFVDILNQPDFSSYDISTMCGGVIAGSPAPPELIRAIINKINMKDLVVAYGTTENSPVTFAHFPEDTVEQKAESVGRIMPHTEARIMNMEAGTLAKLNTPGELCIRGYCVMLGYWGEPQKTEEAVDQDKWYWTGDVATMNEQGFCKIVGRSKDMIIRGGENIYPAELEDFFHTHPKVQEVQVVGVKDDRMGEEICACIRLKDGEETTVEEIKAFCKGKISHFKIPKYIVFVTNYPLTISGKIQKFKLREQMERHLNL

• Full Name: acyl-CoA synthetase family member 2
• Calculated Molecular Weight: 615 aa, 68 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC012053
• Gene Symbol: ACSF2
• Gene ID (NCBI): 80221
• RRID: AB_2878222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96CM8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IP,IHC,IF	Redox Rep; ACSF2-PGK1 interaction promotes ferroptosis in renal tubular epithelial cells of diabetic nephropathy by regulating Keap1/Nrf2 signaling; Authors - Xinran Liu
rat	WB,IP	Prostaglandins Other Lipid Mediat; Targeted Lipidomics Analysis of Lysine 179 Acetylation of ACSF2 in Rat Hepatic Stellate Cells; Authors - Meili Wang
mouse,rat	WB,IHC	Life Sci; ACSF2-mediated ferroptosis is involved in ulcerative colitis; Authors - Lianxiang Luo
human	WB,IF	Free Radic Biol Med; Inhibition of ACSF2 protects against renal ischemia/reperfusion injury via mediating mitophagy in proximal tubular cells; Authors - Haoyu Shi ; KD Validated
human	IHC	Clin Cancer Res; Biomarker identification and risk prediction model development for differentiated thyroid carcinoma lung metastasis based on primary lesion proteomics; Authors - Xiaoqi Peng
human,mouse	WB,IHC,IF	Diabetologia; ACSF2 and lysine lactylation contribute to renal tubule injury in diabetes; Authors - Jingfang Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Dawei (Verified Customer) (10-02-2025)

Works as intended, however there were quite a few non-specific bands near the actual band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

ACSF2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:16140-1-AP)