ACSL3 Polyclonal antibody

ACSL3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30214-1-AP

Synonyms

ACS3, ACSL3, FACL3, LACS 3, LACS3, PRO2194

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Validation Data Gallery

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  WB analysis using 30214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30214-1-AP (ACSL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30214-1-AP (ACSL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of LNCaP using 30214-1-AP

  IP result of anti-ACSL3 (IP:30214-1-AP, 4ug; Detection:30214-1-AP 1:4000) with LNCaP cells lysate 1360 ug.

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Tested Applications

• Positive WB detected in: DU 145 cells, HuH-7 cells, LNCaP cells, MKN-45 cells, mouse kidney tissue
• Positive IP detected in: LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30214-1-AP targets ACSL3 in WB, IP, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSL3 fusion protein Ag33005
• Full Name: acyl-CoA synthetase long-chain family member 3
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC041692
• Gene Symbol: ACSL3
• Gene ID (NCBI): 2181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.