Tested Applications
| Positive WB detected in | BV-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, COLO 320 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, RAW 264.7 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22401-1-AP targets ACSL4/FACL4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, zebrafish, hamster, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18085 Product name: Recombinant human ACSL4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 143-236 aa of BC034959 Sequence: LGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMY Predict reactive species |
| Full Name | acyl-CoA synthetase long-chain family member 4 |
| Calculated Molecular Weight | 711 aa, 79 kDa |
| Observed Molecular Weight | 79 kDa, 74 kDa |
| GenBank Accession Number | BC034959 |
| Gene Symbol | ACSL4 |
| Gene ID (NCBI) | 2182 |
| RRID | AB_2832995 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60488 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ACSL4/FACL4 antibody 22401-1-AP | Download protocol |
| IP protocol for ACSL4/FACL4 antibody 22401-1-AP | Download protocol |
| WB protocol for ACSL4/FACL4 antibody 22401-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ACSL4/FACL4 Polyclonal antibody (Cat# 22401-1-AP) has accumulated 744 citations. Publications span high-impact journals including Nature, Nat Commun, Cell Res, Adv Sci, Cell Death Dis, Redox Biol, Autophagy, ACS Nano, Biomaterials, EMBO J, Phytomedicine, and Cancer Res. Key research fields include ferroptosis mechanisms, cancer biology, lipid metabolism, nanomedicine, cardiovascular disease, neurodegeneration, osteoarthritis, liver disease, and inflammation. The antibody is predominantly used for WB, IHC, IF, IP, and CoIP across human, mouse, rat, and other species.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Bio-barrier-adaptable biomimetic nanomedicines combined with ultrasound for enhanced cancer therapy | ||
J Hematol Oncol METTL16-SENP3-LTF axis confers ferroptosis resistance and facilitates tumorigenesis in hepatocellular carcinoma | ||
Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
Exploration (Beijing) A pH responsive nanocomposite for combination sonodynamic-immunotherapy with ferroptosis and calcium ion overload via SLC7A11/ACSL4/LPCAT3 pathway | ||
Reviews
What customers say
One verified user (Yingjian Li) reviewed this product in June 2025 for Western Blot on H9C2 cells at a 1:5000 dilution (lot 00170295). The reviewer gave a perfect 5-star rating and included an image of the result, though no written comments were provided. Overall, the single available review indicates strong satisfaction with the antibody's performance in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
YINGJIAN (Verified Customer) (06-24-2025) |
 |
