ACSL6 Polyclonal antibody

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  WB analysis using 13847-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13847-1-AP (ACSL6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13847-1-AP

  IP result of anti-ACSL6 (IP:13847-1-AP, 4ug; Detection:13847-1-AP 1:2000) with mouse brain tissue lysate 2120 ug.

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  IHC staining of human urothelial carcinoma using 13847-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13847-1-AP (ACSL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 13847-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13847-1-AP (ACSL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACSL6 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13847-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

ACSL 6, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.3, FACL6

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human pancreas cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13847-1-AP targets ACSL6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACSL6 fusion protein Ag4817
• Full Name: acyl-CoA synthetase long-chain family member 6
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC047453
• Gene Symbol: ACSL6
• Gene ID (NCBI): 23305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKU0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACSL6 (Acyl-CoA Synthetase Long Chain Family Member 6) is a long-chain lipoyl-coenzyme A synthetase that is mainly responsible for catalyzing the binding of long-chain fatty acids to coenzyme A to form acyl-coenzyme A (Acyl-CoA). ACSL6 is involved in fatty acid metabolic transporter and the synthesis of cell membrane phospholipids, which affects the cell structure and function. ACSL6 plays an extremely important role in the pathogenesis of cancers such as hepatocellular carcinoma, lung cancer and neuroblastoma, suggesting that it may serve as a biomarker and therapeutic target for cancer therapy.