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ACSM3 Polyclonal antibody

ACSM3 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10168-2-AP

Synonyms

ACSM3, Butyrate CoA ligase 3, Protein SA homolog, SA, SAH



Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10168-2-AP targets ACSM3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSM3 fusion protein Ag0224
Full Name acyl-CoA synthetase medium-chain family member 3
Calculated molecular weight 49 kDa, 66 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC002790
Gene symbol ACSM3
Gene ID (NCBI) 6296
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SA hypertension-associated rat homolog (SAH, synonym: SA) is expressed at 10-fold greater levels in the kidney of the spontaneously hypertensive rat than in the corresponding wild-type strain. The gene is linked to blood pressure levels in a number of crosses involving the spontaneously hypertensive rat and other strains of genetically hypertensive rats. Human SAH gene polymorphism may be associated with the renal prognosis of immunoglobulin A nephropathy through its effect on blood pressure. Furthermore, variation in SAH has a role in predisposition.

Protocols

Product Specific Protocols
WB protocol for ACSM3 antibody 10168-2-APDownload protocol
IHC protocol for ACSM3 antibody 10168-2-APDownload protocol
IF protocol for ACSM3 antibody 10168-2-APDownload protocol
FC protocol for ACSM3 antibody 10168-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Eur J Cell Biol

Proteomic and cell biological profiling of the renal phenotype of the mdx-4cv mouse model of Duchenne muscular dystrophy.

Authors - Paul Dowling
mouse,humanIHC

Cell

Organoid Models of Human and Mouse Ductal Pancreatic Cancer.

Authors - Sylvia F Boj