smooth muscle actin Polyclonal antibody

smooth muscle actin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 23081-1-AP


a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, ACTA2 specific, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, smooth muscle actin, α SMA, α-sma

Tested Applications

Positive WB detected inmouse heart tissue, mouse brown adipose tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, mouse skeletal muscle tissue, human colon tissue, human liver cancer tissue, stromal tumor tissue, human hysteromyoma tissue, human liver tissue, human small intestine tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23081-1-AP is smooth muscle actin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen smooth muscle actin fusion protein Ag19481
Full Name actin, alpha 2, smooth muscle, aorta
Calculated Molecular Weight 377 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC017554
Gene Symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody is specific to the ACTA2. It selectively stains the vascular smooth muscle but not cardiac or skeletal muscle.


Product Specific Protocols
WB protocol for smooth muscle actin antibody 23081-1-APDownload protocol
IHC protocol for smooth muscle actin antibody 23081-1-APDownload protocol
IP protocol for smooth muscle actin antibody 23081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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