smooth muscle actin Polyclonal antibody

smooth muscle actin Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23081-1-AP

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, ACTA2 specific, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, α SMA



Tested Applications

Positive WB detected inmouse heart tissue, mouse brown adipose tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, mouse skeletal muscle tissue, human colon tissue, human liver cancer tissue, stromal tumor tissue, human hysteromyoma tissue, human liver tissue, human small intestine tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23081-1-AP is smooth muscle actin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen smooth muscle actin fusion protein Ag19481
Full Name actin, alpha 2, smooth muscle, aorta
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC017554
Gene symbol ACTA2
Gene ID (NCBI) 59
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody is specific to the ACTA2. It selectively stains the vascular smooth muscle but not cardiac or skeletal muscle.

Protocols

Product Specific Protocols
WB protocol for smooth muscle actin antibody 23081-1-APDownload protocol
IHC protocol for smooth muscle actin antibody 23081-1-APDownload protocol
IP protocol for smooth muscle actin antibody 23081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Vasc Cell

Effect of the vascular endothelial growth factor expression level on angiopoietin-2-mediated nasopharyngeal carcinoma growth.

Authors - Hai-Hong Chen
humanWB

Biochim Biophys Acta

Inhibitory effects of long noncoding RNA MEG3 on hepatic stellate cells activation and liver fibrogenesis.

Authors - Yong He
mouseIHC

JCI Insight

Long noncoding RNA Malat1 regulates differential activation of macrophages and response to lung injury.

Authors - Huachun Cui
human, mouseWB

Exp Mol Pathol

The anti-fibrotic effects of microRNA-153 by targeting TGFBR-2 in pulmonary fibrosis.

Authors - Chunlian Liang
humanWB

Life Sci

Reticulocalbin 2 enhances osteogenic differentiation of human vascular smooth muscle cells in diabetic conditions.

Authors - Zhihui Chang
ratIHC

J Alzheimers Dis

Locus Coeruleus Degeneration Induces Forebrain Vascular Pathology in a Transgenic Rat Model of Alzheimer's Disease.

Authors - Sarah C Kelly