Tested Applications
| Positive WB detected in | mouse colon tissue, mouse kidney tissue, mouse stomach, rat colon, mouse lung, rat lung |
| Positive IHC detected in | mouse heart tissue, human colon tissue, human colon cancer tissue, human heart tissue, human hepatocirrhosis tissue, human liver cancer tissue, mouse colon tissue, mouse liver tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:3000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55135-1-AP targets Alpha smooth muscle actin specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_001613 |
| Gene Symbol | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_10949628 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Alpha smooth muscle actin specific antibody 55135-1-AP | Download protocol |
| IHC protocol for Alpha smooth muscle actin specific antibody 55135-1-AP | Download protocol |
| WB protocol for Alpha smooth muscle actin specific antibody 55135-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The 55135-1-AP Alpha smooth muscle actin specific Polyclonal antibody has 325 total citations. Publications appear in high-impact journals including Cell, Nature Communications, Nature Cell Biology, Cell Research, Molecular Cancer, Autophagy, Theranostics, Advanced Science, Biomaterials, ACS Nano, Cell Death & Disease, and Frontiers in Pharmacology, among others. Research fields span fibrosis (hepatic, renal, pulmonary, cardiac), cancer biology and cancer-associated fibroblasts, cardiovascular disease, diabetic complications, epithelial-mesenchymal transition, wound healing, and nanomedicine-based drug delivery.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther 2-APQC, a small-molecule activator of Sirtuin-3 (SIRT3), alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis | ||
Cell Sensory neurons drive immune exclusion by stimulating a dense extracellular matrix in the breast cancer tumor microenvironment. | ||
Mol Cancer Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts. | ||
Cell Res α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Reviews
What customers say
This antibody has received 6 reviews with an average rating of approximately 4.7 out of 5. Most users employed it for Western Blot at a 1:1000 dilution, with one reviewer also confirming successful IHC-P performance. Tested samples included mouse lung tissue, pancreas (pancreatitis), A549 cells, cardiac fibroblasts, and lung fibroblasts. Reviewers consistently described the product as "excellent" or "good," with one noting it "works great" across both WB and IHC applications. Overall, users report reliable performance and strong signal across diverse tissue and cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
P (Verified Customer) (01-29-2026) | Good
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S (Verified Customer) (01-06-2026) | Excellent
 |
Zach (Verified Customer) (09-12-2025) | The antibody works great with pancreas samples with pancreatitis, both WB and IHC-P.
|
S (Verified Customer) (09-23-2024) | Excelllent
 |
PK (Verified Customer) (03-22-2022) |
 |
S (Verified Customer) (12-01-2021) | Excellent
 |
