Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11032-1-AP

Synonyms

ACTC, ACTC1, ACTC1 cardiac muscle 1, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha actin, Alpha cardiac actin, CMD1R, CMH11



Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, mouse thymus tissue
Positive IP detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

11032-1-AP targets Alpha Actin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Actin fusion protein Ag1471
Full Name actin, alpha, cardiac muscle 1
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC009978
Gene symbol ACTC1
Gene ID (NCBI) 70
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Alpha Actin antibody 11032-1-APDownload protocol
IP protocol for Alpha Actin antibody 11032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Stem Cells Dev

A model of early human embryonic stem cell differentiation reveals inter- and intracellular changes on transition to squamous epithelium.

Authors - Galat Vasiliy V