Alpha cardiac muscle actin specific Polyclonal antibody

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  WB analysis using 17522-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17522-1-AP (Actin-cardiac-muscle-1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Alpha cardiac muscle actin specific Polyclonal Antibody for WB, ELISA

Cat No. 17522-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Actin-cardiac-muscle-1-Specific, ACTC1, Alpha actin, ACTC, ACTC 1

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17522-1-AP targets Alpha cardiac muscle actin specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha, cardiac muscle 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_005159
• Gene Symbol: ACTC1
• Gene ID (NCBI): 70
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P68032
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.