ACTL7B Polyclonal antibody

ACTL7B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13537-1-AP

Synonyms

Actin like 7 beta, actin like 7B, Actin like protein 7B, ACTL7B

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Validation Data Gallery

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  WB analysis of human testis using 13537-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13537-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13537-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 13537-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13537-1-AP (ACTL7B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13537-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13537-1-AP (ACTL7B antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human testis tissue, human liver tissue, mouse testis tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13537-1-AP targets ACTL7B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACTL7B fusion protein Ag4460
• Full Name: actin-like 7B
• Calculated Molecular Weight: 415 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC033789
• Gene Symbol: ACTL7B
• Gene ID (NCBI): 10880
• RRID: AB_2223776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTL7B is also named as actin-like-7-beta. The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. The ACTL7B gene is expressed predominantly in the testis, however, its exact function is not known. Diseases associated with ACTL7B include Dysautonomia and Hypertensive Nephropathy. An important paralog of this gene is ACTL7A. ACTL7B can be detected as 45 kDa.

Publications

Species	Application	Title
		Cell Rep; The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.; Authors - Brian P Hermann
human,mouse	WB	Development; Actl7b deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis; Authors - Gina E Merges