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ACTN4 Monoclonal antibody

ACTN4 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1E12B8

Cat no : 66628-1-Ig

Synonyms

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4



Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, K-562 cells, pig brain tissue, mouse brain tissue, rat brain tissue, chicken brain tissue
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Sample-dependent, check data in validation data gallery

Product Information

66628-1-Ig targets ACTN4 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACTN4 fusion protein Ag13549
Full Name actinin, alpha 4
Calculated molecular weight 105 kDa
Observed molecular weight 105 and 80 kDa
GenBank accession numberBC005033
Gene symbol ACTN4
Gene ID (NCBI) 81
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 105 and 80 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

Protocols

Product Specific Protocols
WB protocol for ACTN4 antibody 66628-1-IgDownload protocol
IHC protocol for ACTN4 antibody 66628-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IF

J Physiol Biochem

Dysregulated expression of ACTN4 contributes to endothelial cell injury via the activation of the p38-MAPK/p53 apoptosis pathway in preeclampsia.

Authors - Jianlin Zhao
  • KD Validated