|Positive WB detected in||PC-3 cells, mouse spleen tissue, human liver tissue|
|Positive IP detected in||mouse spleen tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Sample-dependent, check data in validation data gallery|
13822-1-AP targets ARP3/ARP3B in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARP3/ARP3B fusion protein Ag4922|
|Full Name||ARP3 actin-related protein 3 homolog (yeast)|
|Calculated molecular weight||418 aa 47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC044590|
|Gene ID (NCBI)||10096|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.
The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling.
Cell Commun Signal
Regulation of MRTF-A by JMY via a nucleation-independent mechanism.
Fluoride altered rat's blood testis barrier by affecting the F-actin via IL-1α.
Mol Cell Biol
MRTF-A activation by competition with WH2 domain proteins for actin binding.
Int J Biol Sci
Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.
WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.