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  • KD/KO Validated

ARP3/ARP3B Polyclonal antibody

ARP3/ARP3B Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 13822-1-AP

Synonyms

Actin like protein 3, Actin related protein 3, ACTR3, ACTR3B, Arp11, ARP3, Arp4



Tested Applications

Positive WB detected inPC-3 cells, mouse spleen tissue, human liver tissue
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

13822-1-AP targets ARP3/ARP3B in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARP3/ARP3B fusion protein Ag4922
Full Name ARP3 actin-related protein 3 homolog (yeast)
Calculated molecular weight 418 aa 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC044590
Gene symbol ACTR3
Gene ID (NCBI) 10096
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

Protocols

Product Specific Protocols
WB protocol for ARP3/ARP3B antibody 13822-1-APDownload protocol
IP protocol for ARP3/ARP3B antibody 13822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Microbiol

The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling.

Authors - Lei He
IF

Cell Commun Signal

Regulation of MRTF-A by JMY via a nucleation-independent mechanism.

Authors - Franziska Kluge
  • KD Validated
ratWB

Chemosphere

Fluoride altered rat's blood testis barrier by affecting the F-actin via IL-1α.

Authors - Xinjin He
mouseWB,IF

Mol Cell Biol

MRTF-A activation by competition with WH2 domain proteins for actin binding.

Authors - Julia Weissbach
  • KD Validated
humanWB

Int J Biol Sci

Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.

Authors - Xin Dong
humanIP

EMBO Rep

WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.

Authors - Kailiang Zhao