ACTR3B Monoclonal antibody

ACTR3B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G12C6

Cat no : 68037-1-Ig

Synonyms

Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, ACTR3B, ARP11, ARP3 beta, ARP3BETA, ARP4, DKFZp686O24114

---

Validation Data Gallery

• 

  WB analysis using 68037-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68037-1-Ig

  Brain tissues were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

---

Tested Applications

• Positive WB detected in: MOLT-4 cells, pig brain tissue, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68037-1-Ig targets ACTR3B in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ACTR3B fusion protein Ag10218
• Full Name: ARP3 actin-related protein 3 homolog B (yeast)
• Calculated Molecular Weight: 418 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC008682
• Gene Symbol: ACTR3B
• Gene ID (NCBI): 57180
• RRID: AB_2918779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.