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Arp5/ACTR5 Polyclonal antibody

Arp5/ACTR5 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 21505-1-AP

Synonyms

Actin related protein 5, ACTR5, Arp5, hARP5, Sarcoma antigen NY SAR 16



Tested Applications

Positive WB detected inmouse cerebellum tissue, HEK-293 cells, human skeletal muscle tissue, mouse heart tissue, mouse ovary tissue
Positive IP detected inHEK-293 cells
Positive IF detected inA549 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21505-1-AP is Arp5/ACTR5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Arp5/ACTR5 fusion protein Ag15976
Full Name ARP5 actin-related protein 5 homolog (yeast)
Calculated molecular weight 607 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC038402
Gene symbol ACTR5
Gene ID (NCBI) 79913
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)

Protocols

Product Specific Protocols
WB protocol for Arp5/ACTR5 antibody 21505-1-APDownload protocol
IF protocol for Arp5/ACTR5 antibody 21505-1-APDownload protocol
IP protocol for Arp5/ACTR5 antibody 21505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Activation of the CARD8 Inflammasome Requires a Disordered Region.

Authors - Ashley J Chui
ratWB,IF

J Cell Biol

Arp5 is a key regulator of myocardin in smooth muscle cells.

Authors - Tsuyoshi Morita
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