Validation Data Gallery
|Positive WB detected in||mouse cerebellum tissue, HEK-293 cells, human skeletal muscle tissue, mouse heart tissue, mouse ovary tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IF detected in||A549 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21505-1-AP is Arp5/ACTR5 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Arp5/ACTR5 fusion protein Ag15976|
|Full Name||ARP5 actin-related protein 5 homolog (yeast)|
|Calculated molecular weight||607 aa, 68 kDa|
|Observed molecular weight||68 kDa|
|GenBank accession number||BC038402|
|Gene ID (NCBI)||79913|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)
LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.
J Cell Biol
Arp5 is a key regulator of myocardin in smooth muscle cells.
Actin-related protein 5 functions as a novel modulator of MyoD and MyoG in skeletal muscle and in rhabdomyosarcoma.
Activation of the CARD8 Inflammasome Requires a Disordered Region.