Tested Applications
| Positive WB detected in | A549 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17334-1-AP targets ACTR8 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11367 Product name: Recombinant human ACTR8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-329 aa of BC032744 Sequence: MILMKMGFSGIVVHQESVCATYGSGLSSTCIVDVGDQKTSVCCVEDGVSHRNTRIFSWNQDISGLQDHEFQIRHPDSPALLYQFRLGDEKLQAPMALFYPATFGIVGQKMTTLQHRSQGDPEDPHDEHYLLATQSKQEQSAKATADRKSASKPIGFEGDLRGQSSDLPERLHSQEVDLGSAQGDGLMAGNDSEEALTALMSRKTAISLFEGKALGLDKAILHSIDCCSSDDTKKKMYSSILVVGGGLMFHKAQEFLQHRILNKMPPSFRRIIENVDVITRPKDMDPRLIAWKGGAVLACLDTTQELWIYQREWQRFGVRMLRERAAFVW Predict reactive species |
| Full Name | ARP8 actin-related protein 8 homolog (yeast) |
| Calculated Molecular Weight | 329 aa, 37 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC032744 |
| Gene Symbol | ACTR8 |
| Gene ID (NCBI) | 93973 |
| RRID | AB_3076387 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H981 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACTR8 antibody 17334-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACTR8 polyclonal antibody (Cat# 17334-1-AP) has 1 citation to date. It was cited in Cell (IF 45.1) in a 2024 study by Jun Ho Lee et al., titled "TGF-β and RAS jointly unmask primed enhancers to drive metastasis." The research falls within the fields of cancer biology and metastasis, specifically examining how TGF-β and RAS signaling cooperatively regulate enhancer activation to promote tumor metastasis. The antibody was used for Western blot analysis in mouse samples.
| Species | Application | Title |
|---|---|---|

