ACTRT2 Polyclonal antibody

• 

  WB analysis using 16992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16992-1-AP (ACTRT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

ACTRT2 Polyclonal Antibody for WB, ELISA

Cat No. 16992-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, CoIP, ELISA

Synonyms

actin related protein T2, Actin-related protein M2, Actin-related protein T2, ARPM2, ARP-T2

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

16992-1-AP targets ACTRT2 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACTRT2 fusion protein Ag10597
• Full Name: actin-related protein T2
• Calculated Molecular Weight: 376 aa, 42 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC029499
• Gene Symbol: ACTRT2
• Gene ID (NCBI): 140625
• RRID: AB_2878338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TDY3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Hum Reprod; Deletion of ACTRT1 is associated with male infertility as sperm acrosomal ultrastructural defects and fertilization failure in human; Authors - Qi Zhang
mouse,human	WB	Development; Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice.; Authors - Xiao-Zhen Zhang
human	CoIP	Cell Rep; The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice.; Authors - Xiao-Zhen Zhang