ACVR1C Polyclonal antibody

ACVR1C Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12610-1-AP

Synonyms

activin A receptor, type IC, Activin receptor like kinase 7, Activin receptor type 1C, Activin receptor type IC, ACTR IC, ACVR1C, ACVRLK7, ALK 7, ALK7

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Validation Data Gallery

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  WB analysis of mouse testis using 12610-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12610-1-AP (ACVR1C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 12610-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12610-1-AP (ACVR1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12610-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12610-1-AP (ACVR1C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12610-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12610-1-AP (ACVR1C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 12610-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12610-1-AP (ACVR1C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 12610-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12610-1-AP (ACVR1C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12610-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12610-1-AP (ACVR1C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12610-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12610-1-AP (ACVR1C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 12610-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12610-1-AP (ACVR1C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 12610-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12610-1-AP (ACVR1C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human brain tissue, human kidney tissue, human skeletal muscle tissue
• Positive IHC detected in: human pancreas tissue, human pancreas cancer tissue, rat pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12610-1-AP targets ACVR1C in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACVR1C fusion protein Ag3162
• Full Name: activin A receptor, type IC
• Calculated Molecular Weight: 493 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC022530
• Gene Symbol: ACVR1C
• Gene ID (NCBI): 130399
• RRID: AB_2222281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Exp Biol Med (Maywood); Epstein-Barr virus microRNA BART10-3p promotes dedifferentiation and proliferation of nasopharyngeal carcinoma by targeting ALK7.; Authors - Wei-Jie Luo
hamster	WB,IF	Animals (Basel); The Photoperiod Regulates Granulosa Cell Apoptosis through the FSH-Nodal/ALK7 Signaling Pathway in Phodopus sungorus; Authors - Yan Qi