ACY3 Polyclonal antibody

ACY3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16786-1-AP

Synonyms

Aspartoacylase-2, ASPA2, Aminoacylase-3, Acylase III, ACY-3

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Validation Data Gallery

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  WB analysis of mouse liver using 16786-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16786-1-AP (ACY3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16786-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16786-1-AP (ACY3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 16786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16786-1-AP (ACY3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16786-1-AP targets ACY3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACY3 fusion protein Ag10220
• Full Name: aspartoacylase (aminocyclase) 3
• Calculated Molecular Weight: 319 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC008689
• Gene Symbol: ACY3
• Gene ID (NCBI): 91703
• RRID: AB_2222751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; Comparison of spinocerebellar ataxia type 3 mouse models identifies early gain-of-function, cell-autonomous transcriptional changes in oligodendrocytes.; Authors - Biswarathan Ramani