ACYP2 Polyclonal antibody

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  WB analysis using 12302-1-AP

  mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 12302-1-AP (ACYP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 12302-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12302-1-AP (ACYP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human medulloblastoma using 12302-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12302-1-AP (ACYP2 antibody) at dilution of 1:50 (under 10x lens).

ACYP2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12302-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Acylphosphatase 2, acylphosphatase 2, muscle type, ACYM, ACYP, ACYP2

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human skeletal muscle tissue
• Positive IHC detected in: human medulloblastoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12302-1-AP targets ACYP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2948

  Product name: Recombinant human ACYP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-99 aa of BC012290

  Sequence: MSTAQSLKSVDYEVFGRVQGVCFRMYTEDEARKIGVVGWVKNTSKGTVTGQVQGPEDKVNSMKSWLSKVGSPSSRIDRTNFSNEKTISKLEYSNFSIRY

• Full Name: acylphosphatase 2, muscle type
• Calculated Molecular Weight: 99 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC012290
• Gene Symbol: ACYP2
• Gene ID (NCBI): 98
• RRID: AB_10597999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14621
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.