ACYP2 Polyclonal antibody

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• ACYP2 Polyclonal antibody (12302-1-AP)

• 

  WB analysis using 12302-1-AP

  mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 12302-1-AP (ACYP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12302-1-AP

  WB result of ACYP2 antibody (12302-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACYP2 transfected HepG2 cells.

• 

  WB analysis of human skeletal muscle using 12302-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12302-1-AP (ACYP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human medulloblastoma using 12302-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12302-1-AP (ACYP2 antibody) at dilution of 1:50 (under 10x lens).

• KD/KO Validated

ACYP2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12302-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Acylphosphatase-2, ACYM, ACYP, EC:3.6.1.7

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, HepG2 cells, human skeletal muscle tissue
• Positive IHC detected in: human medulloblastoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12302-1-AP targets ACYP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2948

  Product name: Recombinant human ACYP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-99 aa of BC012290

  Sequence: MSTAQSLKSVDYEVFGRVQGVCFRMYTEDEARKIGVVGWVKNTSKGTVTGQVQGPEDKVNSMKSWLSKVGSPSSRIDRTNFSNEKTISKLEYSNFSIRY

• Full Name: acylphosphatase 2, muscle type
• Calculated Molecular Weight: 99 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC012290
• Gene Symbol: ACYP2
• Gene ID (NCBI): 98
• RRID: AB_10597999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14621
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACYP2, also named muscle-type acylphosphatase (ACYM), is a small cytoplasmic hydrolase (EC 3.6.1.7) encoded by the ACYP2 gene at human chromosome 2p16.2, with UniProt ID P14621. It catalyzes hydrolysis of acyl phosphate intermediates of ion pumps, especially sarcoplasmic reticulum Ca²⁺/Mg²⁺-ATPase, regulating intracellular calcium homeostasis and skeletal muscle differentiation. Widely expressed in muscle, brain and tumor tissues, ACYP2 exerts dual oncogenic or tumor-suppressive effects across malignancies. In glioma, it boosts Ca²⁺ efflux to activate c-Myc/STAT3 and drives temozolomide resistance; in hepatocellular carcinoma, it inhibits KCNN4/ERK and TERT to suppress tumor progression. It also participates in glycolysis, cell apoptosis and ion transport, serving as a potential prognostic biomarker for multiple cancers. (PMID: 32517717, 39267048)