ADA Polyclonal antibody

ADA Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (2)

Cat no : 13328-1-AP

Synonyms

ADA, Adenosine aminohydrolase, adenosine deaminase

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Validation Data Gallery

• 

  WB analysis of HeLa using 13328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13328-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 13328-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse small intestine using 13328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 13328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, Sp2/0 cells
• Positive IHC detected in: human stomach tissue, human cervical cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse small intestine tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 13328-1-AP is ADA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADA fusion protein Ag4128
• Full Name: adenosine deaminase
• Calculated Molecular Weight: 363 aa, 41 kDa
• Observed Molecular Weight: 45-47 kDa
• GenBank Accession Number: BC040226
• Gene Symbol: ADA
• Gene ID (NCBI): 100
• RRID: AB_2877939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IHC	Front Cell Dev Biol; Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.; Authors - Xiaoting Chen
mouse	WB	Eur J Pharmacol; Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection.; Authors - Gerald Wölkart

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Neta (Verified Customer) (07-28-2022)

antibody worked, signal slightly different than reported but overall OK.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse small intestine

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with NaCit antigen retrieval ph=6.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain (neurons) at 3dpf

ADA Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (neurons) at 3dpf (Cat no:13328-1-AP)