ADA Monoclonal antibody, PBS Only

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  WB analysis using 67870-1-Ig (same clone as 67870-1-PBS)

  Jurkat and MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67870-1-Ig (same clone as 67870-1-PBS)

  WB result of ADA antibody (67870-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADA transfected Jurkat cells. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67870-1-Ig (same clone as 67870-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67870-1-Ig (same clone as 67870-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 67870-1-Ig (same clone as 67870-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit spleen using 67870-1-Ig (same clone as 67870-1-PBS)

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67870-1-Ig (same clone as 67870-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67870-1-Ig (ADA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 67870-1-Ig (same clone as 67870-1-PBS)

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ADA (67870-1-Ig, Clone:2C4E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67870-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 67870-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 67870-2-Ig against Human ADA was performed. The affinity constant is 12.8 nM.

• Featured Product
• KD/KO Validated

ADA Monoclonal Antibody for WB, FC (Intra), ELISA

Cat No. 67870-1-PBS

Clone No.2C4E9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), ELISA

Synonyms

2C4E9, ADA1, EC:3.5.4.4

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Product Information

67870-1-PBS targets ADA in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31326

  Product name: Recombinant human ADA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-363 aa of BC040226

  Sequence: MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL

• Full Name: adenosine deaminase
• Calculated Molecular Weight: 363 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC040226
• Gene Symbol: ADA
• Gene ID (NCBI): 100
• RRID: AB_2918628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00813
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.