ADAL Polyclonal antibody

ADAL Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27369-1-AP

Synonyms

ADAL, adenosine deaminase like

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Validation Data Gallery

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  WB analysis using 27369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27369-1-AP (ADAL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 27369-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 27369-1-AP (ADAL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 27369-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 27369-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 27369-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27369-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, LNCaP cells, K-562 cells
• Positive IHC detected in: human heart tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

27369-1-AP targets ADAL in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAL fusion protein Ag25949
• Full Name: adenosine deaminase-like
• Calculated molecular weight: 267 aa, 30 kDa
• Observed molecular weight: 38 kDa
• GenBank accession number: BC075857
• Gene symbol: ADAL
• Gene ID (NCBI): 161823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.