ADAM17 Polyclonal antibody

ADAM17 Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29948-1-AP

Synonyms

ADAM 17, ADAM17, CD156b, cSVP, Snake venom like protease, TACE, TNF alpha convertase, TNF alpha converting enzyme

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Validation Data Gallery

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  WB analysis using 29948-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29948-1-AP (ADAM17 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 29948-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 29948-1-AP (ADAM17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of HCT 116 using 29948-1-AP

  1X10^6 HCT 116 cells were intracellularly stained with 0.4 ug Anti-Human ADAM17 (29948-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HUVEC cells
• Positive FC detected in: HCT 116 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29948-1-AP targets ADAM17 in WB, FC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAM17 fusion protein Ag32418
• Full Name: ADAM metallopeptidase domain 17
• Calculated Molecular Weight: 824 aa, 93 kDa
• Observed Molecular Weight: 90-110 kDa
• GenBank Accession Number: BC136783
• Gene Symbol: ADAM17
• Gene ID (NCBI): 6868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

Publications

Species	Application	Title
mouse	WB	Theranostics; Neutrophil-like pH-responsive pro-efferocytic nanoparticles improve neurological recovery by promoting erythrophagocytosis after intracerebral hemorrhage; Authors - Linfeng Fan