ADAM17 Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP01169-3

  Sandwich ELISA standard curve of MP01169-3, Human ADAM17 Recombinant Matched Antibody Pair - PBS only. 84292-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32418. 84292-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

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  Sample test of MP01169-3

  The mean ADAM17 concentration was determined to be 18.51 ng/mL in THP-1 cell extract based on a 4.4 mg/mL extract load and 13.76 ng/mL in A549 cell extract based on a 2.7 mg/mL extract load.

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  Sample test of MP01169-3

  Serum of six individual healthy human donors was measured. The human ADAM17 concentration of detected samples was determined to be 1,898.9 pg/mL with a range of 21.5 - 8,096.2 ng/mL.

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  Cytometric bead array standard curve of MP01169-2

  Cytometric bead array standard curve of MP01169-2, ADAM17 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84292-3-PBS. Detection antibody: 84292-1-PBS. Standard: Ag32418. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 84292-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 84292-3-PBS against Human ADAM17 was performed. The affinity constant is 1.06 nM.

ADAM17 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84292-3-PBS

Clone No.241305E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Snake venom-like protease, EC:3.4.24.86, Disintegrin and metalloproteinase domain-containing protein 17, cSVP, ADAM 17

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Product Information

84292-3-PBS targets ADAM17 as part of a matched antibody pair:

MP01169-2: 84292-3-PBS capture and 84292-1-PBS detection (validated in Cytometric bead array)

MP01169-3: 84292-3-PBS capture and 84292-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ADAM17 fusion protein Ag32418
• Full Name: ADAM metallopeptidase domain 17
• Calculated Molecular Weight: 824 aa, 93 kDa
• GenBank Accession Number: BC136783
• Gene Symbol: ADAM17
• Gene ID (NCBI): 6868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78536
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.