ADAM28 Polyclonal antibody

• Featured Product
• KD/KO Validated

ADAM28 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 22234-1-AP

Synonyms

eMDCII, eMDC II, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 28, ADAM23

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Validation Data Gallery

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  WB analysis of mouse lung using 22234-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22234-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 22234-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 22234-1-AP

  IP result of anti-ADAM28 (IP:22234-1-AP, 4ug; Detection:22234-1-AP 1:800) with mouse lung tissue lysate 3600ug.

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Tested Applications

• Positive WB detected in: mouse lung tissue, BxPC-3 cells, PC-13 cells, mouse spleen tissue
• Positive IP detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below

Product Information

22234-1-AP targets ADAM28 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAM28 fusion protein Ag17586
• Full Name: ADAM metallopeptidase domain 28
• Calculated Molecular Weight: 775 aa, 87 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC136478
• Gene Symbol: ADAM28
• Gene ID (NCBI): 10863
• RRID: AB_2879043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAM28 belongs to a family of cell surface and secreted glycoproteins that possess both proteolytic and adhesive properties. ADAM28 is predominantly expressed by carcinoma cells in the breast carcinoma tissues as protein bands of 42 kDa and 55/57 kDa, which correspond to the active forms of ADAM28s and ADAM28m, respectively.In human non-small cell lung carcinomas and breast carcinomas, ADAM28 is overexpressed predominantly by carcinoma cells, and the expression correlates with carcinoma cell proliferation and lymph node metastasis(PMID:19601836).

Publications

Species	Application	Title
mouse	WB	Nat Commun; A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility; Authors - Daiji Kiyozumi
human	WB	Cancer Lett; ADAM28 promotes tumor growth and dissemination of acute myeloid leukemia through IGFBP-3 degradation and IGF-I-induced cell proliferation.; Authors - Jia-Min Zhang ; KO Validated
mouse	WB	Biol Reprod; ADGRG2 is dispensable for lumicrine signalling regulating epididymal initial segment differentiation and gene expression†; Authors - Daiji Kiyozumi
human	WB	World J Gastroenterol; PMID: 31602160; Authors - Li Wei ; KD Validated
mouse	WB	Mol Cell Neurosci; Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.; Authors - Zhen-Huang Chen
mouse	WB	J Reprod Dev; Busulfan administration replicated the characteristics of the epididymal initial segment observed in mice lacking testis-epididymis lumicrine signaling; Authors - Daiji Kiyozumi