ADAM33 Polyclonal antibody

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  WB analysis using 22860-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22860-1-AP (ADAM33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ADAM33 Polyclonal Antibody for WB, ELISA

Cat No. 22860-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

UNQ873/PRO1891, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 33, C20orf153, ADAM 33

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Tested Applications

• Positive WB detected in: MCF-7 cells, SK-BR-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22860-1-AP targets ADAM33 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18852

  Product name: Recombinant human ADAM33 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 30-364 aa of BC125113

  Sequence: VLQGHIPGQPVTPHWVLDGQPWRTVSLEEPVSKPDMGLVALEAEGQELLLELEKNHRLLAPGYIETHYGPDGQPVVLAPNHTDHCHYQGRVRGFPDSWVVLCTCSGMSGLITLSRNASYYLRPWPPRGSKDFSTHEIFRMEQLLTWKGTCGHRDPGNKAGMTSLPGGPQSRGRREARRTRKYLELYIVADHTLFLTRHRNLNHTKQRLLEVANYVDQLLRTLDIQVALTGLEVWTERDRSRRQLRAFFRKGGGACLSNAPDPGLPVPPALCGNGFVEAGEECDCGPGQECRDLCCFAHNCSLRPGAQCAHGDCCVRCLLKPAGALCRQAMGDCDL

• Full Name: ADAM metallopeptidase domain 33
• Calculated Molecular Weight: 813 aa, 88 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC125113
• Gene Symbol: ADAM33
• Gene ID (NCBI): 80332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BZ11
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAM33 is a member of the 'a disintegrin and metalloprotease domain' family of proteins. Endogenous ADAM33 is detected in bronchus tissue, bronchial smooth muscle cells, and MRC-5 fibroblasts, consistent with a role in the pathophysiology of asthma(PMID:12535637). ADAM33 is a novel tumour suppressor gene that may be useful as a molecular marker for invasive lobular carcinoma of the breast(PMID:19267929)