ADAMTS1 Polyclonal antibody

ADAMTS1 Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC,IF,ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12749-1-AP

Synonyms

ADAM TS 1, ADAM TS1, ADAMTS 1, ADAMTS1, C3 C5, KIAA1346, METH 1, METH1

---

Validation Data Gallery

• 

  IHC staining of human liver cancer using 12749-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12749-1-AP (ADAMTS1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12749-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12749-1-AP (ADAMTS1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12749-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12749-1-AP (ADAMTS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 12749-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 12749-1-AP (ADAMTS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, HEK-293 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

12749-1-AP targets ADAMTS1 in IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAMTS1 fusion protein Ag3485
• Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 1
• Calculated Molecular Weight: 967 aa, 105 kDa
• GenBank Accession Number: BC036515
• Gene Symbol: ADAMTS1
• Gene ID (NCBI): 9510
• RRID: AB_2877879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC	Front Aging Neurosci; Induction of A Disintegrin and Metalloproteinase with Thrombospondin motifs 1 by a rare variant or cognitive activities reduces hippocampal amyloid-β and consequent Alzheimer's disease risk; Authors - Yunjie Qiu
human	IHC	NPJ Precis Oncol; Identification of novel prognostic and predictive biomarkers in salivary duct carcinoma via comprehensive molecular profiling; Authors - Shinji Kohsaka
mouse	WB	Front Genet; Identification of polyunsaturated fatty acids related key modules and genes in metabolic dysfunction-associated fatty liver disease using WGCNA analysis; Authors - Cheng Xiao
rat	WB	JOR Spine; Dynamics of N6-methyladenosine modification during aging and their potential roles in the degeneration of intervertebral disc; Authors - Libangxi Liu