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  • KD/KO Validated

ADAMTS12 Polyclonal antibody

ADAMTS12 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 24934-1-AP

Synonyms

ADAM TS 12, ADAM TS12, ADAMTS 12, ADAMTS12, PRO4389



Tested Applications

Positive WB detected inmouse placenta tissue, NIH/3T3 cells
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24934-1-AP is ADAMTS12 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAMTS12 fusion protein Ag20712
Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 12
Calculated Molecular Weight 178 kDa
Observed Molecular Weight 155 kDa
GenBank Accession NumberBC058841
Gene Symbol ADAMTS12
Gene ID (NCBI) 81792
RRIDAB_2879806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAMTS12(A disintegrin and metalloproteinase with thrombospondin motifs 12) is also named as AT12, ADAM-TS12, PRO4389 and belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. ADAMTS7 and ADAMTS12 appear to be potent negative regulators of endplate cartilage development(PMID:22247065). This protein has 3 isoforms produced by alternative splicing. The full length protein has 14 glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for ADAMTS12 antibody 24934-1-APDownload protocol
IF protocol for ADAMTS12 antibody 24934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells.

Authors - Tania Fontanil
humanWB,IF

Hum Mol Genet

Epigenetic changes in reterm birth placenta suggest a role for ADAMTS genes in spontaneous preterm birth.

Authors - Sneha Mani
  • KD Validated
humanWB

Cell Signal

ADAMTS12 promotes oxaliplatin chemoresistance and angiogenesis in gastric cancer through VEGF upregulation

Authors - Yingming Jiang
humanWB

Sci Rep

ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer.

Authors - Yangming Hou
  • KD Validated
humanWB

Int J Mol Med

Overexpression of PRDM13 inhibits glioma cells via Rho and GTP enzyme activation protein.

Authors - Linna Zhang
humanWB

Hepatobiliary Pancreat Dis Int

ADAMTS12 promotes migration and epithelial-mesenchymal transition and predicts poor prognosis for pancreatic cancer.

Authors - Rui-Zhe He
  • KD Validated